Tag

Part:BBa_K1033241:Design

Designed by: Anton Berglund   Group: iGEM13_Uppsala   (2013-08-23)


Signal peptide from Lactococcus lactis usp45


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed according to the Freiburg [1] standard to enable fusion with other proteins. Only the 3' end designed according to this standard as a signal peptide always is fused to the 5' part of a protein. The 5' end is also designed with a B0034 RBS [2]. Illegal restriction sites were removed prior to synthesis.


Source

Synthesized based on the signal peptide from usp45 protein from the Lactococcus lactis MG1363 genome.

References